Zona pellucida3/16/2023 Developmental ability of zona-free mouse embryos is influenced by cell association at the 4-cell stage. Time-lapse monitoring of zona pellucida-free embryos obtained through in vitro fertilization: a retrospective case series. 2016 105(5):1232–5.īodri D, Kato R, Kondo M, Hosomi N, Katsumata Y, Kawachiya S, et al. Live birth of twins derived from zona-free oocytes. Pregnancy and live birth following the transfer of vitrified-warmed blastocysts derived from zona- and corona-cell-free oocytes. OA-5 - Growing zona pellucida-free (ZPF) oocytes from ICSI to biopsy: it works. Pregnancy following fertilization of zona-free, coronal cell intact human ova: case report. Stanger JD, Stevenson K, Lakmaker A, Woolcott R. Role of diagnostic intracytoplasmic sperm injection (ICSI) in the management of genetically determined zona pellucida-free oocytes during in vitro fertilization: a case report. Metwalley A, Brasha N, Esteves SC, Fawzy M, Brasha H, Hellani A, et al. Developmental potential of zona pellucida-free oocytes obtained following mild in vitro fertilization. Ueno S, Bodri D, Uchiyama K, Okimura T, Okuno T, Kobayashi T, et al. Intracytoplasmic sperm injection into zona-free human oocytes results in normal fertilization and blastocyst development. Zona-free embryo culture: is it a viable option to improve pregnancy rates? Reprod Biomed Online. The molecular mechanisms mediating mammalian fertilization. Mammalian egg coat modifications and the block to polyspermy 2020 87(3):326-40. Mammalian sperm-egg interaction: identification of a glycoprotein in mouse egg zonae pellucidae possessing receptor activity for sperm. Structure and function of the mammalian egg zona pellucida. Wassarman P, Chen J, Cohen N, Litscher E, Liu C, Qi H, et al. These data demonstrate that MSHG can substitute the function of zona pellucida and rescue human ZP-free embryos during assisted reproductive technology. A ZP-free blastocyst cultured in MSHG with the highest score was successfully implanted after day 5 transplantation and developed normally. Compared to ZP-free embryos, the proportion of tetrahedrally arranged blastomeres at the 4-cell stage increased significantly in embryos cultured with MSHG in humans. MSHG improves blastocysts formation rate by maintaining blastomere spatial arrangement at early stages. Human and mouse ZP-free embryos cultured with MSHG showed a blastocyst formation rate similar to ZP-intact embryos. The first clinical application of MSHG was reported, and the pregnancy outcome was continuously followed up. The developmental process and the blastocyst formation rate of embryos were analyzed in both mouse and human. To handle cases with missing ZP, we applied modified sodium hyaluronate gel (MSHG) to embryo culture to determine if it can function as a substitute for human zona pellucida. Existing attempts to rescue ZP-free embryos are not widely used due to operational complexity and high technical requirements. Zona pellucida-free (ZP-free) embryos often fail to achieve good developmental outcomes and are routinely discarded in assisted reproductive laboratories.
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